Hieff Trans® Polyethylenimine Linear(PEI) MW40000(rapid lysis) 线性PEI转染试剂（速溶型）（40816ES）

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Hieff Trans® Polyethylenimine Linear(PEI) MW40000(rapid lysis) 线性PEI转染试剂（速溶型）（40816ES）
Hieff Trans®Polyethylenimine Linear (PEI) MW40000（rapid dissolving ） 线性PEI转染试剂（速溶型）MW40000
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产品介绍
PEI 40000是一种分子量为40000的高电荷阳离子聚合物，容易结合带负电荷的核酸分子，形成复合物，并使该复合物进入细胞中。PEI 40000是一种瞬时转染试剂，细胞毒性低，转染效率高，在HEK293和CHO等细胞中基因表达效率较高。目前已经验证线性PEI转染试剂广泛适用于多种细胞系包括HEK-293、HEK293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2和Hela细胞等，转染效率高达80%~90%。
PEI 40000与PEI 25000转染试剂相比，具有很多优点。包括：1. PEI 40000较易溶解，可直接在水中溶解，PEI 25000需要先把水调成弱酸性助其溶解，溶解后再用NaOH把pH调至中性；2. PEI 40000操作简便，更易于使用，且比PEI 25000的转染效果好；3. PEI 25000含有4-11%的丙酰基残留，其能阻止聚合物主链与DNA结合。相较于PEI 25000，PEI 40000是完全脱落的结构，因此其性能始终高效如一。
本产品为速溶型，溶解迅速，配制方便。
点击查看100+种细胞转染案例集锦
产品特色
转染效率高：适合转染DNA，细胞密度在70%~80%时，转染效率可以达到80%以上；
应用范围广：既适合贴壁细胞，也适合悬浮细胞转染；而且可满足瞬时转染和稳定转染。
低毒性：转染细胞形态良好并表达大量的目的蛋白；
操作简便：直接用蒸馏水配置溶液，以及可以直接将复合物加入到含血清的培养基中；
性价比高：相同规格，是竞品poly**的产品价格的1/3，适用于大规模的转染。
应用案例
PEI 40000转染GFP质粒到贴壁细胞Hek-293，72h后，转染效率达到90%（左图）；
PEI 40000转染GFP质粒到悬浮细胞CHO-K1，72h后，转染效率达到80%（右图）
存储条件
室温密封保存，有效期2年。储存液在4 ºC保存，有效期3个月。
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【应用案例】细胞转染 | RAW264.7小鼠单核巨噬细胞DNA转染案例(6孔板)
10907
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【应用案例】细胞转染 | AGS人胃腺癌细胞DNA转染案例
19999
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【应用案例】细胞转染 | HepG2人肝癌细胞DNA转染案例
12235
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【应用案例】细胞转染 | MC38小鼠结肠癌细胞DNA转染案例
7358
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【应用案例】细胞转染 | Hela人宫颈癌细胞DNA转染案例(96孔板)
18135
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【应用案例】细胞转染 | Jurkat人T淋巴细胞白血病细胞DNA转染案例
18984
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【应用案例】细胞转染 | iPAM原代猪肺泡巨噬细胞DNA转染案例
19796
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【应用案例】细胞转染 | PMHs原代小鼠肝脏细胞DNA转染案例
10460
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【应用案例】细胞转染 | mNSC原代小鼠神经干细胞mRNA转染案例
12580
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【应用案例】细胞转染 | BMDM原代骨髓来源巨噬细胞siRNA转染案例
20531
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相关产品
[40818ES]Hieff Trans®293 Transfection Reagent 293细胞专用转染试剂
[40817ES]Hieff Trans® Transfection Reagent for Common Cell Line 常见细胞系转染试剂
[40815ES]Hieff Trans®Polyethylenimine Linear(PEI) MW25000 线性PEI转染试剂
[40810ES]Hieff Trans® Cell Insect Transfection Reagent 昆虫细胞转染试剂
[40809ES]Hieff Trans® mRNA Transfection Reagent mRNA转染试剂
[40808ES]Hieff Trans® Universal Transfection Reagent通用型转染试剂
[40806ES]Hieff Trans® in vitro siRNA/miRNA Transfection Reagent siRNA/miRNA转染试剂
[40805ES]Hieff Trans® Suspension Cell-Free Liposomal Transfection Reagent 悬浮细胞专用脂质体核酸转染试剂
[40804ES]Hieff Trans®Polybrene(hexadimethrine bromide) 聚凝胺(10 mg/mL)
[40803ES]Hieff Trans®Calcium Phosphate Cell Transfection Kit 磷酸钙法细胞转染试剂
[40802ES]Hieff Trans® Liposomal 2000 Transfection Reagent 脂质体核酸转染试剂
[40801ES]Hieff Trans® LipoBooster 3000 Transfection Reagent Lipo3000转染试剂
[40130ES]Fetal Bovine Serum Gold 胎牛血清（特级）
[40807ES]Hieff Trans®RNAiBoost Transfection Reagent转染试剂
[40828ES]Hieff Trans® UltraProFect-CHO Transfection Reagent Kit CHO瞬转表达试剂盒
产品应用
【应用案例】细胞转染 | NIH-3T3小鼠胚胎成纤维细胞DNA转染案例
【应用案例】细胞转染 | HEK293人胚肾细胞DNA转染案例(15K大片段)
【应用案例】细胞转染 | sf9昆虫卵巢细胞DNA转染案例(质粒DNA 6738bp)
【应用案例】细胞转染 | AC16人心肌细胞DNA转染案例(DNA 1563bp)
【应用案例】细胞转染 | RAW264.7小鼠单核巨噬细胞siRNA转染案例(西南医科大学)
【应用案例】细胞转染 | RAW264.7小鼠单核巨噬细胞DNA转染案例(6孔板)
【应用案例】细胞转染 | AGS人胃腺癌细胞DNA转染案例
【应用案例】细胞转染 | HepG2人肝癌细胞DNA转染案例
【应用案例】细胞转染 | MC38小鼠结肠癌细胞DNA转染案例
【应用案例】细胞转染 | Hela人宫颈癌细胞DNA转染案例(96孔板)
【应用案例】细胞转染 | Jurkat人T淋巴细胞白血病细胞DNA转染案例
【应用案例】细胞转染 | iPAM原代猪肺泡巨噬细胞DNA转染案例
【应用案例】细胞转染 | PMHs原代小鼠肝脏细胞DNA转染案例
【应用案例】细胞转染 | mNSC原代小鼠神经干细胞mRNA转染案例
【应用案例】细胞转染 | BMDM原代骨髓来源巨噬细胞siRNA转染案例
下载资源
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